OECD 408/OCSPP 870,31: Oral toxicity study (repeated dose, rodents, 90-day)

The OECD 408: Repeated Dose 90-Day Oral Toxicity Study is used to determine the toxicity of an orally ingested chemical over a prolonged time period. It is often carried out as a follow-on study to acute or repeated dose 28-day oral toxicity studies to determine the sub-chronic oral toxicity of the test substance. The test is performed on rats and involves dividing the animals into sub-groups and administering a different fixed dose of the test substance to the groups daily, through gavage or via their food/water, over a period of 90 days. Throughout this period, clinical and functional assessments are performed to determine the toxicity and thus health hazard of the chemical. Endocrine related measurements, such as thyroid function, are important to consider.